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KMID : 0355420140380040227
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Journal of Korean Academy of Oral Health
2014 Volume.38 No. 4 p.227 ~ p.231
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Photodynamic bactericidal effect against Enterococcus faecalis by erythrosine concentration and LED irradiation times
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Lee Si-Young
Lee Min-Sun
Ma Deuk-Sang
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Abstract
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Objectives: The purpose of this study was to provide photodynamic bactericidal effect against Enterococcusfaecalis by erythrosine concentrations and LED irradiation times.
Methods: Erythrosine was used as a photosensitizer and green LED (3 Watt, 520-530 nm) was used aslight source. E. faecalis ATCC 1943 and E. faecalis ATCC 29212 were used in this study. Approximately105 CFU of bacteria were added in wells of a 96-well microtitration plate. For examining the effects ofconcentrations of erythrosine, 0, 0.625, 1.25, 2.5, 5, and 10 M of erythrosine were added in wellscontaining bacteria. The irradiation time with LED was 30 sec. In another set of experiment, the effect ofirradiation time for killing of bacteria was investigated by increasing irradiation time from 0 to 30 s with10 M of erythrosine final concentration. After irradiation, each sample was serially diluted with PBS and50 l of diluents was spread on duplicate blood agar plates. The plates were incubated for 72 h at 37oCunder aerobic conditions and the number of CFU was determined. The experiments were repeated fourtimes. The results were analyzed using one-way ANOVA, and Tukey’s multiple comparison at a significance evel of 0.05.
Results: When the erythrosine concentrations were more than 2.5 M, E. faecalis ATCC 29212 wassignificantly decreased (P<0.05). The more erythrosine concentrations increased, the more E. faecalisATCC 1943 decreased statistically significantly (P<0.05). In another set of experiment, when LED irradiationtime was more than 20 s, E. faecalis ATCC 1943 decreased significantly (P<0.05), and if theirradiation times was more than 5 s, E. faecalis ATCC 29212 decreased significantly (P<0.05).
Conclusions: PDT using erythrosine and green LED was found to be an effective method in killing E. faecalis.
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KEYWORD
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Enterococcus faecalis, Erythrosine, LED, Photodynamic therapy
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